Cloning and characterization of the human homolog of mouse Jak2.

Characterization of the Properties of Mouse-Adapted Human Measles Virus (34333)

Cloning and Expression of Human Gamma-Interferon cDNA in E. coli

Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be...

O-3: Identification and Characterization of Repopulating Spermatogonial Stem Cells from The Adult Human Testis

Background: This study was conducted to identify and characterize repopulating spermatogonial stem cells (SSCs) in the adult human testes. Materials and Methods: Testes biopsies from obstructive azoospermic patients and normal segments of human testicular tissue were used. Flow cytometry, real time PCR and immunohistochemical analysis were performed. Purified human spermatogonia were transplant...

Oocyte Enucleation Phase in Mouse Cloning

Mouse oocytes were placed in Hepes-T6 containing 7.5 μg/ml cytochalasin B and 5μg/ml Hoechst prior to enucleation, and enucleation was performed on oocytes using the manipulator. The oocyte held in place by gentle suction of a holding pipette (oocyte diameter approx.70 μm). The metaphase spindle (arrow) is aspirated into an enucleation pipette (1).

Chromosomal Variation in Three Human-Mouse Hybridoma Cell Lines after Various Passaging Intervals as Assessed with Two Different Staining Methods

Objective(s) The main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. Materials and Methods Metaphase spreads from 3 human-mouse cell lines (HF2X653, SPMO-4 and F3B6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. Metaphase chromosome...